FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005304622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50873981
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA12295652.416883789770649TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7678651.5093471847622855TruSeq Adapter, Index 22 (97% over 40bp)
CTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCA1041330.20468812928164595No Hit
GTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGC899050.17672098434757838No Hit
CAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCTTGTCCTCCTCTGT726040.14271342358680364No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC704820.1385423326709974No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAGGAAGTCAG657800.12929988710732113No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT637950.12539808905459943No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCA616600.12120144480142021No Hit
CCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCC612370.12036997851612989No Hit
CCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATG554400.10897515568911346No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA549950.1081004452944227TruSeq Adapter, Index 22 (97% over 39bp)
GGTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGG549740.10805916682635865No Hit
CTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCA519810.10217600230656217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)