FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005304690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36166777
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTAT2193980.6066285641100948TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGGAT937580.2592379188225702TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCGCGTAT631060.17448610364147182TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTTT596470.16492207751882343TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph