FastQCFastQC Report
Wed 11 May 2022
EGAF00005304694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39464977
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCTAGATCTCGTAT10489922.6580327159445702TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCTAGATCGCGTAT3279990.831114129370961TruSeq Adapter, Index 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537220.136125760316546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)