FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38924361
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA1135570.2917376087432752No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA1015440.26087518816301186No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA977040.2510099009717847No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA752240.1932568655398094No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA735990.1890821020799802No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAATGGAGTGGAATGGAATGGAA628200.16138993264398097No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGTGGAATGGAATGGAATGGAATGGAAT600810.15435320826461352No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA566380.14550784790018775RNA PCR Primer, Index 26 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAATGGAGTGGAATGGAATGGAATGGAA505830.12995203697756272No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA495980.12742148804960474No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA414380.10645775276824712No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA410870.10555600386092401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCAC150.002356185668.9995924
GCAACGA301.13350325E-768.99187554
ACGACCG208.523041E-568.9912561
GCGTCAC150.002357422168.99045632
CGGGTGC144250.065.29661
CGGTTAA979600.062.9161341
CGGGTAC136650.062.6896361
CGGAATA841500.062.1759221
GGGAGGC889250.061.062112
CGGCTAA4800.060.6449971
TAGGACC3700.060.5977817
CGGGTTT1486100.060.4636961
AGATCGC304850.059.77655827
CGGGTAA218400.059.661221
CGGGCGC267350.059.6128921
GTGAGTC890500.059.4786319
AACGATC11300.059.2199960
CCGGACT353.2912794E-759.1395444
GACCAGA3800.059.003120
CGGGAAA159000.058.716131
Produced by FastQC (version 0.11.5)