FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44910711
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA1185410.26394817040416035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1059880.23599715444273414RNA PCR Primer, Index 29 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA906330.20180709229920676No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA903320.2011368735623001No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA710930.15829854040832264No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA599960.1335895127556542No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAATGGAGTGGAATGGAATGGAA576750.12842148056841052No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA549140.12227372663950922No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAATGGAGTGGAATGGAATGGAATGGAA477230.10626195608437372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA253.0962601E-669.0002924
GACCGCG150.002356955868.9939229
CGCTTCA150.002357403368.9906131
TTCCGAC150.002357746768.9880815
CGGGTGC229450.066.912291
CGGTTAA1318400.064.889761
CGGGTAC201600.064.294131
CGGCTAA7100.062.968251
CGGGCGC434700.062.9278221
GCGCATC454.3837645E-1061.32621853
CGGGTTT1877150.061.1866761
CGGAATA740850.061.0151181
GCTGTCG7750.060.97655516
CGGGACA1200.060.6496471
AGCGCTA401.1981683E-860.3667931
GGGAGGC1026150.060.353222
TGCTGTC7850.060.1997915
CTGTCGG7800.060.1434517
CGGGAAA197450.060.1340521
CGGTACA1500.060.072041
Produced by FastQC (version 0.11.5)