FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34811314
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA798070.22925592524315513RNA PCR Primer, Index 33 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA792410.22763001706858865No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA690170.19826025527217964No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA490880.1410116262775947No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA352000.10111655078575892No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGA150.002354489669.012151
CGGGTGC203350.066.901721
CGGCTAA4000.065.621941
CGGTTAA1019150.065.0327761
CGGGTAC192100.064.293281
CGGGCGC441650.063.625051
CGGTCAA1250.063.5496641
CGGTACA1850.063.4749871
CGCGCTA1550.062.3348531
CGGTGCA850.060.9491651
GGGAGGC979050.060.839822
CGGGTTT1411050.060.7805671
CGGAATA470800.059.9277421
AGATCGC340200.059.88427727
CTGGCGT1500.059.865631
GTGAGTC954700.059.4804819
CGGGAAA156100.059.362641
GGGCGCG513400.059.1093942
CGGGTAA239600.058.8988761
CGGTAAT219850.058.88011
Produced by FastQC (version 0.11.5)