FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38641814
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA1004880.26004990345432544No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA638660.16527691997068253RNA PCR Primer, Index 37 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA513840.13297512378689055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA1000.069.194691
CCGTCAT150.002356174968.99966450
TCGGACC253.0966603E-668.99876444
CGCGTCC150.002356380568.99814663
ACCGTGC150.002356634568.9962757
CGGCTAA3500.067.2177051
CGGGTGC195050.066.6049961
CGGTTAA1131050.065.056041
CGGGTAC186900.064.474351
CGGGCGC452750.063.7309461
CGGGCAA1050.062.6047251
CGGGTTT1631300.060.287141
CGGCATT3800.060.0901261
CGGCAAT750.059.968741
GGGAGGC862700.059.4530872
AACGATC7200.059.416360
CAAGATC1050.059.14157525
TACGCCG353.2909156E-759.140531
AGATCGC285800.058.8105627
GTGAGTC801450.058.7885619
Produced by FastQC (version 0.11.5)