FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005304722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33834948
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1857140.5488821794553962RNA PCR Primer, Index 38 (97% over 44bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA811780.23992352522604735No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA801980.23702711173074656RNA PCR Primer, Index 38 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA481400.14227892414671361No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA455840.13472460486713325No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)