FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44272234
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1139350.257350916603847RNA PCR Primer, Index 39 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA628690.1420054836175649No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA443580.10019372412966557No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC150.00233084469.188021
CGTACCG150.002356343268.99843654
CGGGTGC218150.066.064021
CGGACAT1000.065.728621
CGGTTAA1241000.064.870041
CGGCTAA4550.064.6261751
CGGGTAC194100.063.9303051
GTCTTCT186000.062.74855452
CGTCTTC186000.062.7483451
TTCTGCT187650.062.7475855
CTTCTGC187550.062.74424754
TCTGCTT188350.062.7159356
CCGTCTT186150.062.69791850
TCTTCTG186200.062.679653
CTGCTTG189050.062.645957
GCCGTCT186800.062.64518749
CGGGCGC442450.062.6280981
TGCCGTC187350.062.62715548
ATGCCGT187850.062.49719647
TATGCCG190300.062.3277946
Produced by FastQC (version 0.11.5)