FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37460690
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA910350.24301474425591202No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA667470.17817877887460162RNA PCR Primer, Index 48 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA557040.1486998771245271No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC150.00235640268.99798660
ACGACCG150.00235640268.99798661
GCGACAA150.00235668968.99586554
CGTACCG150.00235668968.99586554
CGGCTAA3350.067.029511
CGGGTGC192100.066.700171
CGGTTAA1168650.065.491481
CGGGTAC193900.065.1723561
CGGGCGC444550.064.4526751
CGGTACA1150.063.083961
CCGTCTT111000.061.8195450
CGTCTTC111100.061.73283851
GTCTTCT110950.061.72202352
TGCCGTC111650.061.70683348
GCCGTCT111400.061.69046449
TCTGCTT112000.061.66505456
TCTTCTG111000.061.66314753
CTTCTGC112250.061.58918454
CTGCTTG112350.061.56514757
TTCTGCT112000.061.51104755
Produced by FastQC (version 0.11.5)