FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46273374
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA494260.10681304544596208No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGCGGATTACGAGGTTAGGAGATC490190.10593348995904212No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT208.519453E-568.9971545
ATTACCT253.097799E-668.9945361
AACCCGA253.097819E-668.9944565
ACCCGAG253.097819E-668.9944566
GGACACT150.00235709568.992893
CGGGTGC298000.067.010051
CGGCTAA5000.065.778591
CGGTTAA1569850.065.4452441
CGGGTAC306400.065.421531
CGGGCGC827600.065.0573961
CCGTCTT59550.061.80955550
GCCGTCT59950.061.68485349
CTGCTTG59500.061.63345357
GTCTTCT59800.061.55500852
CGTCTTC59850.061.44581251
TCTTCTG59900.061.39465353
TGCCGTC60350.061.3936548
TCTGCTT60450.061.3496856
CAGCGAC454.3837645E-1061.32847260
CTTCTGC60300.061.27345354
Produced by FastQC (version 0.11.5)