FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41397455
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA781230.18871449947828917No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA472860.1142244130708035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTAC301.1070915E-769.227251
CCGCTAT208.5198066E-568.9965569
TCGCTAC150.002356597568.9965569
GACTGCT208.5198066E-568.9965567
CGACTGC208.5204185E-568.99554466
AACGTCC150.002356755568.99538463
CGGCTAA3850.068.32821
CGGCATA1250.066.458161
CGGGTGC236400.066.328141
CGGTTAA1310000.065.076261
GCGCATC750.064.3984953
CGGGTAC213500.064.2013851
CGGGCGC485500.063.4737631
CGGGCAA1000.062.3045271
GCTGTCG7850.061.52335416
CGGAATA544750.061.3482441
CGTGACT454.3837645E-1061.32938461
CTGTCGG7900.061.13300317
AACGATC6950.060.55724360
TGCTGTC8050.060.42335515
Produced by FastQC (version 0.11.5)