FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39414221
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA774320.19645700976812402No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA590170.14973529478103856No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA514420.13051634332694284No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA456890.11592008884305997No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA431100.10937676530509129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACG253.0362662E-669.228011
CGGCAAA500.069.228011
AGCCATC150.00235667568.9959729
CTATAAC150.00235684168.9947560
ACTGGCG150.002357113668.9927449
CGGGTGC241100.065.969041
CGGGCAA800.064.901251
CGGTTAA1268900.064.623361
CGGGTAC212850.064.024121
CTAGAAC600.063.24518660
CGGGCGC469600.062.830021
CTTTGAC1050.062.42381760
CGGAATA641100.061.8527531
CGCGCTA950.061.73323431
CGGGTTT1764950.060.420311
CTGGCGC1150.060.1982651
CGGCTAA4100.059.9413221
CGGGCAC1400.059.338291
GGGAGGC821850.059.2076952
CAGCGAC353.2917342E-759.13835560
Produced by FastQC (version 0.11.5)