FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40745958
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1588320.38981044451083957No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC1118440.2744910304968164No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1030410.25288643354513834No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC631090.15488407463631115No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC525900.12906801700428788No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC462450.11349592025790632No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCATATTAATTCCATTCCCTTCCATTC445750.10939735421118335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGAT208.5210035E-568.9945952
ATAGGTC150.002357138668.9925569
ATTGGGT150.002357299268.9913767
TCGCGTT905550.066.44932649
CTCGCGT886750.066.37977648
AGTAGCG8450.066.14104542
GTTAATT997350.065.8887453
TTAGTAG8650.065.8090940
TAGCGGT8550.065.77128644
GTAGCGG8400.065.71332643
GCGTTAA893150.065.6535851
TAGTAGC8700.065.4272641
GCGTTGA9200.065.2448851
ATAGGAC9100.065.2034316
TAGGACC9050.065.18249517
CGCGTTA907800.065.0960150
CTCGAGT13500.064.9131148
CAATAGG9200.064.86967514
GGACCAG8950.064.7579519
GGTCGCG8800.064.6894948
Produced by FastQC (version 0.11.5)