FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32020844
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC874820.2732032922055396No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC431910.13488401492477836No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC394610.12323535257221827No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC379610.1185509039049689No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC331130.10341076581241893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGAGT150.002355029869.008157
CTGGCGT253.0960855E-669.0008848
TCGCGTT448150.066.5765149
CTCGCGT444050.066.2349448
TAGGACC5100.065.6121317
GCGTTAA443200.065.0947951
GTTAATT501350.065.0310453
GGACCAG5150.064.9790719
GTAGCGG4950.064.81647543
AGTAGCG4900.064.773842
CGCGTTA454500.064.28892550
TAGCGGT5000.064.1687144
CTCGAGT7700.064.0722448
TAGTAGC5050.063.52980441
GGCTAAT2500.063.4730762
CGGGTTA600.063.2452750
ACTCGCG454600.063.1496247
AGGACCA5300.063.14004518
ACACTCG976000.062.74561345
GCCATTC431350.062.7422910
Produced by FastQC (version 0.11.5)