FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32773085
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA662360.20210486745449813No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACC150.002356618668.9963664
CGGCTAA4500.067.597081
CGGGTGC194600.067.055111
CGGGCGC579950.066.89231
CGGTTAA1116500.066.028091
CGGGTAC226250.065.9860841
GTCGACA700.064.070423
GGGCGCG666700.062.8911672
CGGGTTT1308300.060.2215461
CGGTACA750.059.9155921
CGGACGC15350.059.9005741
CGCGGTG665600.059.5427175
CGGGTAT862750.059.4775351
CGGAATA389250.059.4094121
TCGAGCC700.059.14001544
GCGCGGT672400.058.8586354
GGCGCGG687350.058.727913
GGGCGTG663450.058.716592
GGGAGGC719600.058.565112
GTGAGTC668400.058.5262119
Produced by FastQC (version 0.11.5)