FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37209492
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA1002090.26931031469067085No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA741840.19936848371915425No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA660560.17752459506837664No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA476550.12807215965216617No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA447030.12013870009297627No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTACGTCGTTGTATTTTAGTTTGGGTAGTAGAGCGAGAGTTTGTTT410530.11032937509601044No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGTGGAATGGAATGGAATGGAATGGAAT388100.10430134332390241No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC150.002355987769.00104538
CCTCGTA208.5172185E-569.0007641
CTATGCG150.00235631468.99863416
CGGGCAA1100.065.8910141
CGGGTGC202350.065.753791
CGGTTAA1132050.064.915811
CGGCTAA5350.063.8676641
CGGGTAC190450.063.573811
CGGGCGC421000.062.6586951
GCCGTCT20900.062.5607949
AACGATC13600.062.14875460
TGCCGTC21150.062.14753748
ATGCCGT21250.062.0174347
CGTCTTC21100.061.967851
CCGTCTT21100.061.80429550
CGGACTG1900.061.7625121
CTTCTGC21300.061.70971354
GTCTTCT21200.061.51161252
TTCTGCT21450.061.4390255
CTGCTTG21800.061.08561757
Produced by FastQC (version 0.11.5)