FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40061807
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA996710.24879307116626065No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA711940.17771040632291No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA688500.17185944707885992No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA449420.11218165970396693No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAATGGAGTGGAATGGAATGGAA428990.10708203950960074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC229550.065.993141
CGGTTAA1397950.065.566551
CGGGTAC227700.064.4066851
CGGGCGC506750.063.875351
CGGGACA600.063.2923051
CGGTCAA1500.062.1415371
CGGCTAA4600.061.5411341
CGGAATA606600.061.0613371
CGGGTTT1973650.060.051761
CGGATGA166150.059.63361
GGGCGCG581550.058.762562
AGATCGC235600.058.49950427
GAATGGC429950.058.3259755
CGGTACT1850.057.8494761
GTGAGTC665850.057.81810819
CGGGTAT985700.057.7789541
CGCGTCA2450.057.73552331
GGGAGGC688950.057.6895642
TGCCGTC32850.057.6537548
CCGTCTT32800.057.63646350
Produced by FastQC (version 0.11.5)