FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27699909
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA600490.21678410568063597No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA401960.14511239008041507No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA369360.13334339834834835RNA PCR Primer, Index 34 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACA253.0960382E-669.0010160
AGCCGAA208.518173E-568.9991467
GCGACTC150.002356373768.99814655
ACTATTC150.00235645868.9975231
GTCGTCC150.00235645868.9975233
CGGGTGC138000.065.859491
CGGTTAA861500.064.7851
CGGGTAC128100.063.4313471
CGGCTAA2450.063.4005471
CGGGCGC277750.061.4552231
CGGGTTT1415850.060.04491
CGGAATA336900.059.80471
AGATCGC167100.058.9224827
GGGAGGC498350.058.499612
GTGAGTC489100.058.27985419
AACGATC5650.058.009760
GAATGGC344700.057.82873555
CGGAATT342300.057.7923131
CGGGTAA156600.057.6403431
CGGACAA309.103251E-657.530131
Produced by FastQC (version 0.11.5)