FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41753448
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1108630.2655181914557092RNA PCR Primer, Index 40 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA954960.22871404536458884No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA776700.1860205652955895No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA492870.11804294581851062No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA476730.11417739679846321No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAATGGAATGGAATGGAATAGAATGGAA460810.11036453803767296No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT208.517908E-568.9996650
TACCGAC208.520232E-568.9958515
CACCGAA150.002356771168.9952769
CGGGTGC254550.066.08951
CGGGTAC245850.065.304431
CGGTTAA1269500.064.956141
CGGCTAA4050.064.917211
CGGGCGC566250.062.889371
TGCACTT1100.062.72132539
CCGTCTT181700.062.44905550
GTCTTCT181250.062.41390652
GCCGTCT182100.062.38631449
TGCCGTC182850.062.3569148
CTGCTTG183750.062.31269557
CGTCTTC182050.062.30996751
CTTCTGC183050.062.3082254
TCTGCTT183750.062.27737856
TTCTGCT183250.062.24021555
TCTTCTG181800.062.22352253
ATGCCGT183350.062.1492347
Produced by FastQC (version 0.11.5)