FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27876583
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA561740.20150963265476257No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA519300.18628538512055082TruSeq Adapter, Index 16 (97% over 36bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA475540.1705876218760384No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA412370.14792702534596872No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA299790.1075418748416906No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACG301.11209374E-769.182051
CCGACTT150.0023561968.99950450
CCGTCAT208.517955E-568.999550
CGACTTC150.002356206868.99937451
GCCGACT150.002356340968.9983849
ACAGTAC150.002357246168.991717
CGGGTGC125850.065.334021
CGGTTAA776200.064.226471
TCTGCTT87250.064.1358156
TCTTCTG86250.064.118553
TTCTGCT86800.064.11059655
CCGTCTT86450.064.0909250
CTGCTTG87500.064.0685657
GCCGTCT86650.064.061449
TGCCGTC87150.064.0501348
GTCTTCT86350.064.0456252
CGTCTTC86600.063.9797951
CTTCTGC86950.063.96032354
ATGCCGT87550.063.7969147
CTTGAAA88450.063.61672260
Produced by FastQC (version 0.11.5)