FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24903084
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA515120.20684988252860573No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA462430.18569186049406572RNA PCR Primer, Index 42 (100% over 43bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA452020.1815116553435711No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA427120.17151289374440531No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA337080.13535672931111664No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA333250.13381876718562247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA354.0781742E-969.18681
CCGTCTA150.002356202668.9993950
CTATCAT150.0023562468.99911556
AACGACC301.1326847E-768.9989760
CAACGAT150.002356408968.9978643
CGACGCA150.002356427768.9977324
CGACAAC150.002356427768.9977340
GCGAGCC208.519276E-568.9973164
CCACGTG150.002356615468.9963432
CCATTAG150.002357215968.99190510
CGGCTAA2500.066.419331
CGGGTGC125600.065.68891
CGGTTAA707700.064.6994861
CGGGTAC116100.063.078581
CGGGCGC250700.061.5837061
CGGACAG454.274625E-1061.4993741
CCGAGTA454.3837645E-1061.33007432
GTCTTCT75150.061.01155552
TCTGCTT75600.060.9674756
GCCGTCT75600.060.96685849
Produced by FastQC (version 0.11.5)