FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24903084
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC867340.34828618013736773No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC767980.3083875073464797No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCACATTAATTCCATTCCATTCCATTC519080.20844004702389474No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC432500.1736732687405303No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCACATTAATTCCATTCCATTCCATTC408970.16422463980766397No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC380160.15265579154774564No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC321010.12890371329109276No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC309090.12411715753759656No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCATATTAATTCCATTCCCTTCCATTC255810.10272221705552613No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCTA150.002357366568.9907916
TCGCGTT444750.066.73791549
CTCGCGT439350.066.2076448
GCGTTAA438900.065.84309451
GTTAATT484900.065.8361853
GCGTTGA4750.065.3636451
CGCGTTA447600.065.1721750
GTTGATT5700.064.153253
ACTCGCG447200.064.1191247
CGTTGAT5400.063.88421252
CAATAGG6050.063.85950514
CACTCGC544650.063.78983746
TAGGACC5950.063.77300317
GAGTTAA7700.063.61871751
ACACTCG753000.063.39668345
CTCGGGT1150.062.99551448
CGTTAAT495650.062.88402652
GGCGTTA550.062.72268750
GTAGCGG5500.062.71877743
CGAGTTA8050.062.5668550
Produced by FastQC (version 0.11.5)