FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41555955
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC2273200.5470214798336364No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1173810.28246493192130945No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC1170050.2815601277843332No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC815530.19624864835858064No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC735970.17710337784319963No Hit
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC613890.14772612011924646No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC536660.12914153940151296No Hit
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCATTAATTCCATTCCATTCCATTC424330.1021105158093467No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGT150.002356738368.99551449
TCGCGTT1090950.066.5005649
CTCGCGT1078400.066.404548
GTTAATT1199450.066.10277653
GCGTTAA1061450.066.081451
CGCGTTA1085950.065.4685550
ACTCGCG1087700.064.96081547
GCGTTGA12650.064.9028351
CACTCGC1291400.064.82413546
ACACTCG2274000.064.5296645
CGTTAAT1213950.064.29304552
CTCGAGT14600.064.2699448
TAGCGGT9900.063.41966244
CGTTGAT14200.063.40571252
GTTGATT14650.063.3418753
TAGTAGC9900.063.0699941
GTAGCGG9800.063.00938843
CAATAGG10100.062.84528414
TAGGACC9950.062.75501317
TTAGTAG9950.062.752940
Produced by FastQC (version 0.11.5)