FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29386291
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA368950.12555174111629125No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA335090.11402936151418361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT455.456968E-1269.2364041
CGGACAA253.0340616E-669.2364041
TCGGACC253.0968913E-668.9978344
ACTACGC150.002356496968.99724657
GGATTCC150.002356496968.99724655
CAGCGAC208.520992E-568.99454560
CTAAATC150.002356862468.99454560
CACTATA150.002356894368.9943110
CGGCTAA3850.066.538881
CGGGTGC154250.066.296381
CGGTTAA835400.065.162941
CTACACG650.063.9105261
CGGGTAC140700.063.8234631
CACGACG650.063.6857534
CGGGCGC328000.063.4209631
CGGCAAT454.2564352E-1061.5434721
CGGTCAA900.061.5434721
CGGCATT2900.060.8802871
CGGCAAG401.1650627E-860.5818521
CGGGTTT1080650.060.285941
Produced by FastQC (version 0.11.5)