FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37666924
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA805680.21389588382635122No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA666490.17694303893782248RNA PCR Primer, Index 38 (97% over 44bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAAGAATATAAATTTTAGGGTGATTGA650900.1728041291611707No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA629430.16710416810249756No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA558620.1483051814902645No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTACGTCGTTGTATTTTAGTTTGGGTAGTAGAGCGAGAGTTTGTTT391690.10398778514539705No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCC208.517971E-568.99953545
GCAACGA150.002356352368.9983554
CATTCAG150.002356352368.9983568
TAACCGA150.002356352368.9983568
AGCAATC301.132812E-768.99798635
CATCTCG208.5202664E-568.9957838
CGGGTGC222500.066.706031
CGGGTAC239800.065.348951
CGGTTAA1182750.065.301041
CGGGCGC569100.065.172541
AGATCTC650.063.6955227
CCGCTTG650.063.6907856
CGGGCAC1250.063.5251121
CGGCTAA5400.063.2949521
CTTCCGC550.062.7257753
CTAGCAA501.6370905E-1162.097233
ATGCCGC2150.060.9785246
GGGCGCG639000.060.7654652
CGGAATA549450.060.5914881
AACGATC9450.060.23481860
Produced by FastQC (version 0.11.5)