FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37666924
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1735070.4606349061048893No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC967110.2567531131557225No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC725290.1925535517580358No Hit
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC696060.18479342778295355No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC594240.15776175405244133No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC555600.14750341705630116No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTTA150.002356331668.99850551
CTCGCGT846000.066.652148
TCGCGTT854900.066.5684449
GGCGTAT8050.065.996246
GTTAATT1001000.065.8321553
GCGTTAA839050.065.7461551
TGGTCGG5100.065.6128241
CGCGTTA855100.065.25780550
TGGGCGC8150.065.1853941
GCGTTGA8900.065.12218551
ACTCGCG860500.064.7672447
GTCGGCG5600.064.6826643
TAGGACC10800.064.52415517
CTCGAGT19200.064.32519548
CACTCGC1044700.064.2078546
CGGCGTA7800.064.1309345
TAGTAGC10600.064.1132141
ACACTCG2029500.064.06142445
TTAGTAG10750.063.8650940
CGTTAAT1012200.063.7871552
Produced by FastQC (version 0.11.5)