FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32146724
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA784920.24416795938522382No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA569720.17722490167271787No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACG150.002349223769.051071
CGGGCAA1000.069.051071
CCGTAAT253.094934E-669.005220
ATGCCGC150.00235581469.00230447
GGTCGCC150.00235614868.9998345
CGGGTGC182250.066.948281
CGGCTAA4900.066.937271
CGGGCGC564600.066.018011
CGGTTAA1063900.066.003841
CGGGTAC213350.065.782191
CGGCATG650.063.739451
CGGGCAC1300.063.739451
CGGTACA1000.062.145961
CGGTCAA1700.060.9274181
TGCTGTC6400.060.92036415
CGGGCAT5300.060.5825461
CGGGCTC401.1899829E-860.419691
GGGCGCG671000.060.3099332
CGGCTAT750.059.844261
CGGAATA330800.059.4803541
Produced by FastQC (version 0.11.5)