FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33403506
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA350190.10483630071645773TruSeq Adapter, Index 16 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACT208.5167165E-569.00156449
CTCGAGC150.002356068769.0004330
TGCGACC150.002356166568.999764
ATGATCC150.002356404268.9979555
CGGGTGC185850.066.042281
CGGTTAA1084350.065.425211
CGGCATA950.065.41751
CGGGCGC604650.065.2431951
CGGGTAC217000.065.137811
CGGCTAA4200.064.9415741
CGGCAAT750.064.448361
TTCTGCT56550.064.11745555
CTTCTGC56550.064.0564554
TGCCGTC56600.064.0045648
GCCGTCT56400.063.9243249
ATGCCGT56900.063.8490147
TCTGCTT57150.063.806556
CTGCTTG56950.063.78827357
CCGTCTT56250.063.78811350
CGTCTTC56400.063.67963451
Produced by FastQC (version 0.11.5)