FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33893696
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1376930.40624958694383756No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC830200.24494230431523312No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC645250.19037463485835243No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC430510.12701772034539993No Hit
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC388040.11448736661826436No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC369920.10914123971608172No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGAATTAATTCCATTCCATTCCATTC349620.1031519253609875No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACCAAAATAATCTCGATCTCCTAACCTC345970.10207502893753456No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACG208.477819E-569.0653841
GGATACG150.002356406568.997932
TCGCGTT727350.066.7257949
CTCGCGT723100.066.4968648
TAGCGGT6850.066.477844
TAGTAGC6900.065.995241
GTAGCGG6800.065.9509943
GTTAATT786550.065.92911553
GCGTTAA714250.065.7863151
CGTTGAT7300.065.2176152
TTAGTAG7000.065.0571240
CGCGTTA729300.064.9816150
AGTAGCG6850.064.9660842
TAGGACC7300.064.7430217
CAATAGG7200.064.682814
GGACCAG7150.064.655619
GTTGATT7800.064.5764253
GAGTTAA13950.064.5468451
ACTCGCG736250.064.5313647
GCGTTGA6950.064.5308251
Produced by FastQC (version 0.11.5)