FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35168164
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGCGGATTACGAGGTTAGGAGATC354190.10071324735633058No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACGA208.492949E-569.040532
CATACGC150.002357100168.9928136
CCGTCAT150.002358030868.98595456
CGCTTAC150.00235817768.9848744
GTCACTT150.002358203568.9846864
CGGGTGC196850.067.003381
CGGTTAA1239550.066.444181
CGGCTAA5600.066.100231
CGGGTAC232300.065.873131
CGGGCGC653400.065.614261
CCGAGGC3550.063.15516344
CTGTCGG8100.062.5993417
TGCTGTC8150.062.21529815
CGGCAAT750.060.5295141
GCTGTCG8450.060.41467716
CGGCATG700.059.8643531
GGGCGCG794450.059.5289342
GAATGGC336100.059.26674355
GGAGGCC5650.059.22181739
GTGAGCC2650.058.57372319
Produced by FastQC (version 0.11.5)