FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31225445
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC347140.11117215463222381No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT202100.065.5231449
CTCGCGT200150.064.9382148
GTTAATT224250.063.5930253
TAGCGGT3500.063.0749544
GCCATTC427900.062.72865310
GCGTTAA202600.062.70413251
GCGTTGA2050.062.2560851
CGCGTTG2000.062.08781450
CAGATTC15700.061.084911
TAGGACC3850.060.92623517
GGACCAG3550.060.2447919
CGCGTTA214900.060.2387650
GCGATAG2350.060.1813246
GGCTAAT3500.060.1354562
TAGTAGC3700.059.6665541
GTTGATT2850.059.3092853
GATAGCT3550.059.275378
TTCACGC463650.059.1681255
ATTCACG470500.058.8999754
AGTAGCG3750.058.87099542
Produced by FastQC (version 0.11.5)