FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31259113
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1711150.5474083669616601No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC1037310.33184242943809694No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC1016710.32525235121034946No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC763050.244104815130231No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTAAATTAATTCCATTCCATTCCATTC583360.18662077839508753No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC498870.15959186046002008No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTC340770.10901460959560816No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCACATTAATTCCATTCCATTCCATTC339450.10859233273829619No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCACTC328230.10500297945114437No Hit
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCATTAATTCCATTCCATTCCATTC323120.10336825616261089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1019350.067.1785649
CTCGCGT985800.066.9233548
GCGTTAA983950.066.7273351
GTTAATT1089100.066.6034553
CGCGTTA998050.066.35538550
CAATAGG6650.065.9099114
ACTCGCG990850.065.5623747
CACTCGC1159500.065.5290346
CGTTAAT1072750.065.5222752
ACACTCG1509550.065.3399645
GCGTTGA10350.064.98234651
CGAGTTA23500.064.8722550
GAGTTAA23750.064.6245551
CGTTGAT11450.064.1621252
CTCGAGT21000.064.0534148
TCGAGTT25550.063.98724449
CGCATTG2750.063.9649550
TAGCGGT6600.063.75601644
GTAGCGG6700.062.80423443
GCATTAA194050.062.74255451
Produced by FastQC (version 0.11.5)