FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35599851
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGATTGTAATGGAATGGAATAGAA967630.2718073173957947No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGATTGTAATGGAATGGAATAGAA781850.21962170572006043No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGATTGTAATGGAATGGAATGGAATGGAA499640.14034890202208994No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGATTGTAATGGAATGGAATGGAATGGAA481620.13528708308357806No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTACGTCGTTGTATTTTAGTTTGGGTAGTAGAGCGAGAGTTTGTTT444890.12496962417061802No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGTGGAATGGAATGGAATGGAATGGAAT417830.1173684687612878No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAATGGAGTGGAATGGAATGGAATGGAA360210.10118300776034146No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT150.002356282468.99885624
ATTCCTA150.002356951868.993928
CGGGTGC172000.066.387591
CGGTTAA1190200.065.541431
CGGGTAC184950.065.32371
CGGGCGC449050.064.613141
CGGCTAA4150.064.065431
CGGGTTT1726050.060.451611
GGGCGCG523350.059.608292
CGGAATG3739500.059.310631
CGGACAT1400.059.192181
GAATGGC416750.058.9905155
GTGAGTC605800.058.6779719
TGGTGGC406650.058.3390127
GCGTGAA426200.058.30188860
CGGAATA384200.058.1830521
GGGAGGC632400.057.961532
CGGGTAT866900.057.8015441
AGATCGC193500.057.69671227
CGGGTAA181350.057.576521
Produced by FastQC (version 0.11.5)