FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005304861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33036130
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC944480.2858930510323092No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCGCGTTAATTCCATTCCATTCCATTC524260.15869292196150092No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCAAATTAATTCCATTCCATTCCATTC393720.11917860839026848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT468450.065.90282449
CTCGCGT455600.065.3393648
CAATAGG12950.065.3189814
TAGGACC12750.065.26221517
GTTAATT494600.064.9413653
GCGTTAA452200.064.8301251
GGACCAG12600.064.6003219
ATAGGAC13150.064.3339216
GCGTTGA4800.063.9419851
GTAGCGG11600.063.9200143
TAGCGGT11800.063.71302844
CGCGTTA467300.063.5174950
AGGACCA13100.063.18778218
GCCATTC549750.062.940310
ACACTCG888000.062.55689645
TTAGTAG12400.062.29734840
AGTAGCG12150.062.15981742
ACTCGCG476250.062.1513647
CACTCGC537700.061.87878846
AGCGGTC12250.061.65304645
Produced by FastQC (version 0.11.5)