FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005304873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61143187
Sequences flagged as poor quality0
Sequence length100
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTC8392301.3725650251106474No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTC6494111.0621150644306454No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTC4466180.7304460593459088No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTACATTCCCCTACACTC2061490.3371577605203994No Hit
CAAAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCATTCCATTC1936680.31674502017698225No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1625940.26592333173604443No Hit
CAACCTATTTATCTATTTATTAACTTTAAATCCAAATTATAAAACCAATT1375200.22491467446732863No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1200760.19638492183928852No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC1161710.18999827405136732No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACC1102560.18032426082075179No Hit
CAAATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTT1069820.17496961681110929No Hit
CAAAAAATTCCATTCCATTACATTCCATTACATTCCATTCCCCTACACTC751160.12285260825543817No Hit
CAACTACGTTTATTTATTTATTTTTTTAATTATTTTACTTTTTTTTAATT701870.11479120314745778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)