FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22590797
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108250.054.0988494
CAAGTCG93350.029.55436594
TAAGTCG27150.024.23572594
GAAGTCG119450.017.43072394
GTTCGAT31000.014.4032251
GCGGTAT22850.013.781181
AAAGTCG45050.013.45837994
TTCGATT33400.012.3832332
GTCGAAT12650.012.2608681
TATACGG19600.011.51020434-35
GTCCGAT16250.010.9907681
GTCGGTT46450.010.9278781
CGGGAAT59400.010.76094151
AATCGTA19050.010.3622055
GTCGGAT28200.010.3333321
GTCGGAG36500.010.3013691
CGGAAAT45450.010.2376241
GTTTATT91850.010.23407651
TTAGCGA24850.010.0241448
CGACCTA10355.456968E-129.9903384
Produced by FastQC (version 0.11.5)