FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22590797
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130400.044.80137694
CAAGTCG97400.026.29876594
TAAGTCG30800.022.12662394
GAAGTCG124550.018.18867994
GCGGTAT22600.012.6858411
AATCGTA18550.012.6684645
GTTCGAT29400.012.62925151
CGAATCG13750.012.3054533
AAAGTCG52550.012.16365394
TCGGATT25450.011.6345772
GTCCGAT15050.011.5548171
TTCGATT32100.011.2741422
GGCGAAT48850.011.1606951
TACCGTA16100.011.0931679
TTAGCGA22200.011.0090088
CGCGGAT27400.010.63503551
CTCGGAT63800.010.5344821
TATACGG18650.010.33243834-35
CGCGTAT4551.1860831E-410.329671
GGGAAAT153400.010.1720981
Produced by FastQC (version 0.11.5)