FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22758605
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG152750.050.7692394
CAAGTCG133850.029.88195894
TAAGTCG35700.028.04201794
GAAGTCG131100.020.32723294
CGTATCG18800.013.03
AAAGTCG50650.012.80552994
GTCCGAT14400.012.4027791
CGCGTAA9150.011.8142081
TTAGCGA18700.011.8128358
GTCGTTT24050.011.3347191
GCCGTAT20900.011.2440191
CGCGTAT4054.6680265E-410.4444451
TACGCGA8350.010.41317474-75
GTCGGTT46700.010.3661671
GTATCGA25450.010.3418464
CCGTATC35000.010.342
CCCGTAT32700.010.2048931
GTCGGGT43350.010.1914651
TAGCGAG24750.010.0646479
GGGAAAT151850.010.0283171
Produced by FastQC (version 0.11.5)