FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21972019
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG130550.045.68594494
CAAGTCG105300.028.25356194
TAAGTCG32200.024.5217494
GAAGTCG118900.018.53910894
AAAGTCG49700.013.90140894
GTTCGAT35350.013.0297031
TATACGG19350.012.38759734-35
TTCGATT37200.012.1290322
AATCGTA20650.012.0629545
CGTATCG20800.011.9759623
TTAGCGA26600.011.8383468
TAGCGAG29900.011.47491559
GTCCGAT16500.011.1090911
GGCGGAT89400.010.9351231
GGCGATT45500.010.5362631
GGCGGTT55700.010.2944351
TCGATTC39250.010.05859853
GGGGAAT147750.09.9884941
GTATACG19250.09.88831134-35
CCGGTTA21550.09.8143852
Produced by FastQC (version 0.11.5)