FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22430305
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120700.057.00745894
CAAGTCG90550.027.30204294
TAAGTCG37300.024.19302794
GAAGTCG135500.020.32619994
GTTTTTT307300.014.3615361
TTTTTTC405500.013.9435262
AAAGTCG49050.013.60652494
CGAAAAT36650.012.8240121
GTCGAAT15800.012.493671
CGCAAAT30050.012.1996681
TTTTTTG435700.012.0601332
GTTTAAT98250.011.8157761
CTCGATT40850.011.5055081
CGCGAAT19200.011.2604171
CGAAGAT82500.011.166061
ACTCGAA42500.011.05882494
GTTTTTC176800.011.0056551
GTTTTCT223000.011.0017941
TTTTTTA424500.010.9500582
GAAAAAT248600.010.8897831
Produced by FastQC (version 0.11.5)