FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22430305
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG142800.047.78991394
CAAGTCG101300.027.884594
TAAGTCG38150.020.5740594
GAAGTCG127200.018.8812994
AAAGTCG58150.014.54858194
GTTTTTT305800.013.217791
CGAAAAT42000.012.4214281
CGAATCG14400.011.753
GTCGATT19150.011.5352481
CGCAAAT31400.011.3757961
GGAAAAT184300.011.271841
CTTTAAT123800.010.89581
CGAAAAC35900.010.8662961
GCGAAAT28200.010.8333331
CGCGGAT22700.010.766521
CGGAAAT44100.010.7641721
GTTTAAT92250.010.7501361
GCCGAAT50100.010.6946111
CGCAAAC41550.010.51985551
GAAAAAT229800.010.45126151
Produced by FastQC (version 0.11.5)