FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22219996
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116200.058.325394
CAAGTCG99700.032.1033194
TAAGTCG34750.024.34532294
GAAGTCG121950.020.50348594
AAAGTCG48150.015.6178694
GTCCGAT14750.014.0203381
TCGGATT23700.013.286922
GGCGAAT69350.012.1312181
CGCGTTT12550.011.9840631
GTCGGAT29700.011.5521891
GTCGTTT20050.011.2518691
ATCGTAT17850.010.2689076
GTCGGTT47200.010.2563551
GCGGTAT23000.010.2173911
CGAATGC65050.09.8985393
AATCGTA24700.09.8947375
GGGAAAT164500.09.5714271
GCGAATG72200.09.4390572
GTCGGAA35750.09.3342661
GGGGAAT141300.09.2469921
Produced by FastQC (version 0.11.5)