FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22219996
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137750.049.26896394
CAAGTCG114900.028.10182694
TAAGTCG32150.026.3141594
GAAGTCG128550.019.01205694
AAAGTCG54450.014.06978894
AATCGTA23100.014.0389615
ATCGTAT18800.013.256
GGCGAAT54650.011.9542531
GCGGTAT24100.011.7012441
TAATCGT25350.011.6804724
GGCGATT32650.011.37212851
TCGGATT24200.011.0702482
TCGTATC20900.010.7942577
TACGCGA6650.010.60150374-75
TTAGCGA20100.010.52238758
GTCCGAT15750.010.4444441
CGCGTTT13800.010.2173911
CGCGGTT37600.010.1251
CGTATCA23300.010.0858368
GAATCGG33100.09.9395764
Produced by FastQC (version 0.11.5)