FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22562048
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125100.051.99680394
CAAGTCG96700.027.26680494
TAAGTCG37350.024.53815394
GAAGTCG138700.020.46719694
AAAGTCG55050.015.28247194
CGAATCG18600.014.4032273
GTCGGTT40650.013.4120541
GGCGAAT38150.012.935781
CGAAGAT81700.012.8286421
GTTTTAC80200.011.8379061
TACGCTA8350.011.8203589
GTCGATT21800.011.2110091
GTTTTTT294450.011.1574121
GTTTTAT155750.011.0446221
CGGTATA13750.010.9381812
GCGGTAT27300.010.8461531
CGGAAAT41900.010.7684961
CGCAAAT29550.010.6565141
GTTTAAT94600.010.6321341
GGAAAAT194100.010.4848021
Produced by FastQC (version 0.11.5)