FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22562048
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG152000.042.91842394
CAAGTCG108050.027.66496894
TAAGTCG42150.022.0782994
GAAGTCG133050.019.1815194
TATACGC9400.017.5000027
CGAATCG17900.015.229053
TACGCTA9300.015.1612919
ATATACG12350.012.9392716
AATCGTA21900.012.8767125
AAAGTCG64600.012.80495494
GGCGAAT37550.012.7669761
CGAAGAT81350.011.901661
GTCGGTT39200.011.751
CGCAAAT30900.011.4077671
CGGAAAT46300.011.369331
AATCGGG36450.011.3470515
CGAAAAT38600.011.2020731
CGCAAAC41700.010.9328541
CGCGAAC12900.010.9302321
GTCGTAT11650.010.8927041
Produced by FastQC (version 0.11.5)