FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22229295
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136800.059.36842794
CAAGTCG108550.030.82819294
TAAGTCG37700.027.4270694
GAAGTCG147850.024.50930294
AAAGTCG53400.016.89887894
GTTTTTT272400.016.1152731
TTTTTTC377350.014.9463372
CGCGAAT9650.014.1243541
GCCGAAT51100.013.8884551
AAATCGA30850.013.8638584
GAAAAAT202050.013.1427881
TTTTTTG396400.012.9475282
GTTTTTC164950.012.850561
GTCGATT21300.012.7981221
CGCGATT7350.012.7891171
GTTTTAC62900.012.70270251
GTTTAAT85400.012.6030451
GTCGAAC50900.012.3732811
AAAAATC199650.012.2885062
TATACGC6552.6921043E-1012.1984747
Produced by FastQC (version 0.11.5)