FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22229295
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG159450.051.3772494
TAAGTCG33300.028.65165394
CAAGTCG130950.027.85185294
GAAGTCG145100.022.4796794
CGCGAAT7800.020.487181
AAAGTCG61250.017.4955194
CGAAAAC31850.015.4945061
GCGAAAT26750.015.4616821
GTTTTTT274400.015.2441691
AAATCGA32150.013.5956464
CGCAATT18900.013.1798941
AAATCCA189400.013.1768754
AAAAATC197000.012.8116752
CGCAAAT29400.012.7891171
CGGAAAT44100.012.682541
CGCAAAC43250.012.6057811
CGAAAAT37050.012.43184951
GGAAAAT161700.012.4112561
GTTTAAT78500.011.6152871
TTTAATC101250.011.60493852
Produced by FastQC (version 0.11.5)