FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22828252
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106300.051.46566894
CAAGTCG89100.028.53759894
TAAGTCG30850.021.93841494
GAAGTCG123800.018.1470194
AAAGTCG52300.014.82791794
GCGGTAT25050.012.0079841
AATCGTA23700.011.3037985
GTCGGTT45050.011.1631531
TTAGCGA19150.011.0443868
GGCGAAT49800.010.66465951
ATCGTAT16000.010.2812516
TCGGATT27650.010.0289342
CGCGGAT22750.09.9164851
TAGCGAG26200.09.8664139
GTTTATT103100.09.6644041
GTCGGAT30300.09.6171621
GTCGGGT49850.09.6168511
CTCGGTT39300.09.56743051
GTCGTTT20700.09.5362331
GTCCGAT16800.09.5119061
Produced by FastQC (version 0.11.5)