FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22828252
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126900.043.03703794
CAAGTCG100000.027.44800294
TAAGTCG30400.022.26315994
GAAGTCG122900.017.017994
GCGGTAT24850.014.3742461
AAAGTCG51700.013.0909194
CGGTATA12450.011.3253022
GTCCGAT13350.011.2659181
TTAGCGA20150.011.1960318
GGCGAAT40050.010.6791521
AATCGTA20400.010.3676485
GTCGAAT11350.010.3524231
CGGGAAT51500.010.2213591
GGCGATT37750.010.2092711
TCGGATT25350.010.1972392
CTCGGAT62050.09.77115251
TAGCGAG27350.09.6234019
GCCGGTT52350.09.6064951
TATACGC7402.5470035E-79.5270277
GGGAAAT151550.09.5209511
Produced by FastQC (version 0.11.5)